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Fig. 4. Association of injected Xlsirts and VegT RNAs with the cytokeratin
filaments in stage VI oocytes. (A) Texas Red-labeled synthetic RNA (red) was
injected into stage VI oocytes, cytokeratin was labeled with FITC-conjugated
antibody (green), and vegetal cortices were observed under a fluorescence
microscope. In Xpat RNA-injected oocytes, particles of Xpat RNA were visible
outside of cytokeratin filaments (1, arrows). In VegT RNA-injected (2,3) and
Xlsirts RNA-injected (4) oocytes, RNA particles were concentrated around and
on the cytokeratin filaments (arrows). Scale bar: 500 nm. (B) Student's
t-test statistical analysis of the number of RNA aggregates
associated with cytokeratin filaments showing the statistically significant
difference (P=0.03) between the number of aggregates in VegT and
Xlsirts versus Xpat RNA-injected samples. Each bar represents the average
(with the standard deviation) number of RNA aggregates counted in eight
independent samples along the 2 µm length of cytokeratin filament in each
experimental group.
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