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Fig. 5. Association of endogenous Xlsirts and VegT RNAs with cytokeratin network in
the oocyte cortex. (A) Texas Red-labeled molecular beacons (red) that
hybridize to VegT, Xlsirts and Xpat RNAs were used to detect the association
of endogenous RNAs with the cytokeratin network that was detected with
FITC-conjugated antibody (green). Xlsirts RNA (1, 2) and VegT mRNA (3, 4)
particles were intimately associated with the cytokeratin filaments (arrows).
VegT mRNA particles were often visible uniformly spaced along the cytokeratin
filament (4, arrows). Xpat mRNA was not associated with the cytokeratin (5).
Scale bars: 2 µm in 1, 3 and 5; 1.2 µm in 2 and 4. (B) Student's
t-test statistical analysis of the number of endogenous RNA
aggregates associated with cytokeratin filaments. Graph shows that the
difference in the number of RNA aggregates in VegT and Xlsirts samples versus
Xpat samples is statistically highly significant (P=0.002). Each bar
represents the average (with the standard deviation) number of RNA aggregates
counted in eight independent samples along the 2 µm length of cytokeratin
filament in each experimental group.
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