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Fig. 9. Oocyte cytokeratin network disintegrated by the injection of antisense
oligonucleotides or anti-pancytokeratin C11 antibody, reconstitutes
spontaneously in cleaving embryos. Cleaving 2-cell stage embryos originating
from control mock-injected oocytes (1), or oocytes injected with antisense
Xlsirts ODNs (2), antisense VegT ODN (3), or anti-pancytokeratin C11 antibody
(4) were produced by host transfer. Fixed embryos were stained with
anti-pancytokeratin C11-FITC antibody and cortices of vegetal blastomeres were
observed under fluorescent microscope. Intricate cytokeratin network
comparable with control (1) reconstitutes in antisense Xlsirts (2) and
antisense VegT embryos (3). In anti-pancytokeratin antibody embryos (4), the
cytokeratin network also reconstitutes but looks different from control. Scale
bars: 10 µm.
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