|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. Premature neurogenesis in Mib1–/– embryos.
(A,B) Sagittal sections of wild-type (A) and
Mib1–/– (B) embryos at E9.5. The
Mib1–/– embryo has a smaller head, with
picnotic cells in the brain and inside the ventricles. Enlarged views of the
forebrain are shown in insets. (C-H) Sagittal and transverse sections of
E9.0-E9.5 brain regions from wild-type (C,E,G) and
Mib1–/– (D,F,H) embryos. Sections were stained
for nestin (C,D; in red) and huC/D (E-H; in red). BrdU incorporation (E,F; in
green) and TUNEL staining (C,D,G,H; in green) were used for the detection of
proliferative and apoptotic cells, respectively. Nuclear DNA was stained with
Hoechst (in blue). (I) Impaired neurosphere formation in
Mib1–/– forebrains. Neural stem cells are
absent in E9.5 (n=3) and E9.75 (n=5)
Mib1–/– forebrains. (J) Expression of
neurogenin 1 (Ngn1), Dll1, Hes5 and Lfng in the
neural tubes of E9.5 wild-type and Mib1–/–
embryos. (K,L) Neurogenin 1 (Ngn1) (K) and Neurod1 (L)
expression in E9.5 wild-type (left) and
Mib1–/– (right) embryos. Note that the
expression of both Ngn1 and Neurod1 in the trigeminal
ganglia is increased in the Mib1–/– embryos
(arrows), and Ngn1 expression is also increased in the neural tube of
the Mib1–/– embryos (asterisk).
|
