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Fig. 2. FOG-1 dose affects germline proliferation. (A) The FOG-1 protein. Red indicates region used to generate FOG-1 antibodies (Ab, inverted Y); black boxes indicate RRM motifs; gray boxes indicate C/H Zn-finger motif. Sites of fog-1 mutations are marked and bracketed by class of molecular lesion. (B-D) Broken lines indicate germlines; small arrowheads indicate sperm; large arrowheads indicate oocytes or oocyte-like cells; arrow indicates distal end; vul indicates vulva. (B) fog-1(q250)/+; fbf-1 fbf-2 Nomarski micrograph. fog-1/+; fbf-1 fbf-2 animals generate more germ cells than fbf-1 fbf-2 animals; only sperm are made. (C) fog-1(q250)/+; fbf-1 fbf-2, DAPI stained. (D) fog-1(q325); fbf-1 fbf-2 Nomarski micrograph. fog-1(q325); fbf-1 fbf-2 animals generate more germ cells than fbf-1 fbf-2 animals, but fog-1(q325) homozygotes only make oocytes. Scale bars: 10 µm.

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