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Fig. 6. FBF represses fog-1 expression in vivo. (A-J) Arrow indicates distal end. Scale bars: 10 µm. (A-E,G-J) Animals or dissected germlines stained with -FOG-1 antibodies. Broken lines indicate germlines in A-D. (A) Wild-type L2. FOG-1 is barely detectable. (B) Wild-type L3. FOG-1 is abundant in proximal germline where cells are destined to become sperm; FOG-1 is low in distal germline where cells remain in mitotic cell cycle. (C,D) L2 fbf-1 fbf-2. FOG-1 is more abundant than in wild type (compare with A) and its distribution is not graded. (D) L3 fbf-1 fbf-2. FOG-1 is more abundant than in wild type (compare with B) and its distribution is not graded. (E) Wild-type adult male germline. MR, mitotic region; TZ, transition zone. FOG-1 levels are low in mitotic region and become high as germ cells begin entry into meiosis in proximal part of mitotic region and transition zone. FOG-1 disappears in more mature stages of meiotic prophase. (F) Wild-type adult male germline hybridized with fog-1 antisense probe. fog-1 mRNA has same pattern as FOG-1 protein (compare with E). Sense probe has no detectable staining (not shown). (G) fog-1(q250) homozygous male germline. No FOG-1 protein is detectable. (H) Wild-type adult hermaphrodite germline. No FOG-1 protein is detectable. (I) Adult gld-3 nos-3 tumorous germline. FOG-1 levels are low. (J) Adult fbf-1 fbf-2 gld-3 nos-3 tumorous germline. FOG-1 levels increase in the absence of FBF (compare with I).

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