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Fig. 1. STAT expression in wild-type egg chambers. (A) Schematic drawing of a
Drosophila ovariole, with stages (S) indicated above. Pre-migratory
and migratory border cells are indicated in blue and polar cells are indicated
in red. (B-H) Confocal micrographs of egg chambers stained with anti-STAT
(green) and anti-FasIII (red) antibody. (B) The germarium through stage 2 of
egg chamber development; (C-F) egg chambers at stages (C) 4-6, (D) 8, (E) 9
and (F) 10. (G,H) High magnification images of the stage 9 border cell cluster
shown in E, with and without FasIII staining to mark the interface between the
two polar cells. Border cell clusters are indicated by the white arrowheads,
stalk cells are indicated by the open arrowhead, and the outer follicle cells
are indicated by the arrow. nc, nurse cells; o, oocyte. Scale bars: in B, 50
µm for B-F; in G, 10 µm for G,H.