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Fig. 2. Overexpression of HOPTUM increases STAT levels in border cells. (A) An ovariole from a c306-GAL4; UAS-mCD8-GFP fly stained with DAPI (blue), showing the pattern of expression of the GAL4 driver (green). (B) An ovariole from a slbo-GAL4; UAS-mCD8-GFP fly stained with an antibody against Armadillo (red), showing the GAL4 pattern of expression (green). Arrow indicates the normal border cell cluster; arrowheads indicate the anterior cells that express the GAL4 driver but do not normally migrate. (C-H) Egg chambers from c306-GAL4; UAS-hopTUM flies stained with antibodies against STAT (green). (C-E) Antibody staining for FASIII (red); (G,H) phalloidin staining of F-actin (red). Arrowheads indicate some of the ectopic border cells that formed. In all examples, anterior is to the left. Scale bars: 50 µm in A,B; in C, 50 µm for C-F; in D, 10 µm for D,E,G,H.





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