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Fig. 3. STAT expression is reduced in stat mutants. (A-C) Outer follicle cells of stat³⁹⁷ mosaic egg chambers. (A) Bright GFP (green) indicates homozygous wild-type cells, intermediate GFP staining indicates heterozygous cells, whereas lack of GFP depicts stat mutant cells. (B) STAT staining (red). (C) Co-localization of GFP and STAT (merged image of A and B). (D) STAT (green) and rhodamine phalloidin (red) staining in slbo^LY6/slbo^E7b mutant egg chambers. (E,F) STAT (green) and FASIII (red) staining of stat¹⁶⁸¹/stat^ts egg chambers, after (E) 0 hours and (F) 2 hours at 29°C. (G,H) STAT (green) and rhodamine phalloidin (red) staining in stat³³⁹¹/stat^ts egg chambers kept at 29°C for (G) 0 hours and (H) 6 hours. Arrowheads indicate border cells with reduced expression of STAT. Scale bars: in A, 50 µm for A-C; in D, 10 µm for D-H.

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