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Fig. 2. Yan is required for the invasive migration of border cells. (A) Comparison
of BC migration in stage 10 egg chambers between wild type (WT) and three
different yan mutant alleles. Ninety-five percent of wild-type BCs
reach the NC-oocyte boundary. Seventy-nine percent of completely mutant
yan443 or yan884 mutant BC clusters
are detectable at the anteriormost pole of the follicular epithelium and do
not show any signs of migration, while approximately 15% of
yan443 or yan884 mutant BCs migrate
less than 25% of the way towards the oocyte. Only 2% of
yan443 or yan884 mutant BCs reach the
NC-oocyte boundary on time. BCs of the weaker yan1 allele
exit from the epithelium but are delayed in their migration to the oocyte.
Sixty-seven percent of mutant BCs migrate less then 25% of the way towards the
oocyte, while 25% reach the NC-oocyte boundary. (B,C) Stage 10 egg chambers
stained with Alexa-568-phalloidin (red) and DAPI (blue). Wild-type cells are
marked by expression of GFP and yan mutant cells by lack of GFP
expression. (B) Wild-type BC clusters reach the NC-oocyte boundary at stage 10
of oogenesis. (C) yan443 mutant BC clusters remain part of
the anterior follicle cell epithelium. (C') High magnification image of
a yan443 mutant BC cluster. Arrows indicate the boundary
between BCs and squamous follicle cells.
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