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Fig. 5. Yan modulates endocytosis. (A-C) Live FM1-43 uptake in Drosophila SL2 cells. FM1-43 membrane incorporation in 20 minutes after yan^RNAi (A), overexpression of yan^ACT (B) and gfp^RNAi control (C). (D) Kinetics of FM1-43 uptake is accelerated in cells overexpressing yan^ACT (red) and decreased in yan^RNAi cells (blue), when compared with a gfp^RNAi control (orange). (E) Twenty-minute FM1-43 uptake after yan^RNAi, gfp^RNAi and MT-Yan^ACT in triplicate. (F,G) Stage 10 egg chamber, stained with anti-DE-Cad (green), Alexa-568-phalloidin (red) and DAPI (blue). slbo-Gal4, UAS-Rab5^(S43N)-expressing BCs (G) show impaired ability to migrate and elevated levels of cortical DE-Cad expression when compared with control egg chambers (F). (H) Overexpression of dominant-negative Rab5 in BCs delays their migration (46% migrate less than 25%, 25% migrate between 25-50%, 15% migrate 50-75% and 14% complete their migration by stage 10 of oogenesis).

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