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Fig. 6. Activated Yan enhances slbo mutant border cell migration defects. (A,B) slbo¹³¹⁰ mutant egg chamber stained with Alexa-568-phalloidin (red) and anti-Yan (green). Arrows indicate BCs. (A) Yan is normally expressed in slbo¹³¹⁰ mutant egg chambers at stage 9. (B) Yan is strongly expressed in border cells of slbo¹³¹⁰ mutant egg chambers that fail to migrate towards the oocyte at stage 10. (C) Statistical representation of BC migration defects. Overexpression of wild-type Yan in BCs has only minor effects on BC migration. By contrast, expression of Yan^ACT specifically in BCs delays their migration; 38% completely failed to migrate, 22% migrated less than 25% and 26% of BC clusters migrate less than 50% towards the NC-oocyte boundary. Interestingly, this phenotype can be enhanced by removing one copy of slbo¹³¹⁰ resulting in 72% of BCs that completely failed to migrate, 17% that migrated less than 25% and 11% that migrated less than 50% towards the oocyte. Expression of Yan and Yan^ACT enhances the BC migration defects of slbo mutant egg chambers. These BC migration defects are significantly stronger than the defects observed in homozygous slbo¹³¹⁰ mutant egg chambers, where 56% of mutant BCs show no migration and 42% migrate less than 25% of the way towards the oocyte.

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