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Fig. 7. Yan expression depends on JAK/STAT and Notch pathways. (A) JAK/STAT and Notch signaling around the anterior and posterior polar cells (green) specify terminal cell populations during oogenesis. (B,C) ß-galactosidase (ß-gal) staining of a Su(H)-lacZ Notch activity reporter. Arrows indicate BCs. (B) ß-Gal is dynamically expressed during early oogenesis, strongly in BCs of stage 9 egg chambers. (C) ß-Gal is strongly expressed in BCs of stage 10 egg chambers. (D-K') Egg chambers are stained with Alexa-568-phalloidin (red) to visualize actin. Arrows indicate BCs and arrowheads mark squamous cells. (D) BCs migrate normally in N^ts egg chambers at the permissive temperature. (E) BCs have migration defects in N^ts egg chambers at the restrictive temperature. (F-H) Slbo expression is shown in green; (F'-K') Yan is shown in green. (F,F') Wild-type control BC cluster expressing Slbo and Yan. (G,G') N^ts BCs have reduced Slbo and Yan expression. (H,H') Slbo and Yan are normally expressed in BCs where Serrate (Ser) is ectopically expressed. (I,J) stat92E mutant follicle cells are positively marked by GFP expression (green, arrows), whereas wild-type cells do not express GFP (arrowhead). (I',J') stat92E mutant BCs do not express Yan (arrows), whereas Yan expression (green) is normal in wild-type cells (arrowhead). (K,K') tai^61G1 mutant BC clones are negatively marked by lack of GFP expression. (K) tai mutant BCs do not express GFP (green). (K') The expression of Yan in tai mutant BCs and squamous follicle cells was similar to that observed in wild-type control egg chambers (F').

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