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Fig. 8. RTKs signaling downregulates Yan. (A) Yan (blue) is strongly expressed in
BCs that delaminate from the follicular epithelium, but expression gradually
decreases as BCs migrate along the increasing gradient of Pvf1 (orange). The
inverse expression levels of Yan and Pvf1 suggest that PVR activity
counteracts Yan expression in migrating BCs. (B-E') Egg chambers are
stained with Alexa-568-phalloidin (red) to visualize actin. Arrows indicate
BCs and arrowheads the squamous follicle cells. (B-E) Anti-Slbo (green) and
actin (red) staining; (B'-E') anti-Yan (green) and actin (red)
staining. (B,B') Slbo and Yan are strongly expressed in wild-type BCs
that delaminate from the follicular epithelium. (C) Slbo is normally expressed
in BCs that ectopically express activated PVR. (C') By contrast, Yan is
undetectable in BCs but is expressed normally in squamous follicle cells where
slbo-Gal4 is inactive. Similarly, expression of either activated EGFR
(D,D') or activated Raf (E,E') in BCs downregulates Yan but not
Slbo. All experiments and stainings were done in parallel, and images were
taken with the same settings, together with experiments in
Fig. 7F-H. The expression
levels of Yan in squamous follicle cells serve as an internal control. (F,G)
ß-Gal staining indicates yan expression in
yanP(lacZ) (F) and
yanP(lacZ)/slbo-Gal4;
UAS-
PVR (G) stage 10 egg chambers. Arrows indicate
BCs; insets show yan mRNA expression by in situ hybridization. (H,I)
Stage 10 egg chamber stained with anti-Slbo antibody (red), anti-ß-Gal
antibody indicating pnt-lacZ expression (green), DAPI (blue) and
Alexa-568-phalloidin (gray). (H) Pnt-lacZ is expressed in posterior cells and
is absent in wild-type BCs. (I) Expression of activated PVR in BCs arrests
their migration. These BCs express Slbo but do not express
pnt-lacZ.
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