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Fig. 2. The yeast two-hybrid test of the interaction between LIN-31 and SMA-3 or SMA-2. (A) An interaction can be detected between SMA-3 and LIN-31 by using a His selection plate (50 mM 3AT): (1) sma-3-AMA:lin-31, (2) sma-3-DME:lin-31, (3) sma-3:lin-31, (4) lin-31:vector, (5) sma-3-AMA:vector, (6) sma-3-DME:vector, (7) sma-3:vector and (8) vector:vector. (B) There was no detectable interaction between SMA-2 and LIN-31 when using the His selection plate (25 mM 3AT). (1) sma-3:vector, (2) sma-3:lin-31, (3) lin-31:vector, (4) vector:vector, (5) sma-2:lin-31, (6) sma-2:vector, (7) sma-2-DID:lin-31 and (8) sma-2-DID:vector. (C) Both MH1 and MH2 domains of SMA-3 can interact with LIN-31, as detected using the His selection plate (25 mM 3AT): (1) sma-3:vector, (2) sma-3:lin-31, (3) lin-31:vector, (4) vector:vector, (5) sma-3 MH2:lin-31, (6) sma-3 MH2: vector, (7) sma-3 MH1:lin-31 and (8) sma-3 MH1:vector. Note, the sma-2 or sma-3 cDNA was inserted into pPDleu vector and lin-31 cDNA was inserted into pPC86 vector. The yeast strain used for all of the tests was Mav203.





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