Supplemental Figure 1
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Fig. S1. Superior view of the skull from a wild-type mouse.
Frontal, parietal and interparietal bones are labeled. Interfrontal (I) and
sagittal (S) are the midline sutures. Coronal (C) and lambdoid (L) are the
transverse sutures. The arrow denotes the junction between the parietal and
interparietal bones.
Supplemental Figure 2
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Fig. S2. Cells from the limb of P1 mice prior to culture.
(A,B) ALP, (C,D) collagen type 1, and (E,F) collagen type II staining are
positive for both mutants and controls. Scale bars: A-F, 100 µm.
Supplemental Figure 3
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Fig. S3. DNA content assay of cultured cells from the limb of
P1 mice. No significant difference was detected between day 1 (after
encapsulation) and day 21 (at harvest) in mutant and control cells. For DNA
assay, lyophilized hydrogel samples were homogenized in 1 ml papainase-PBE
solution [papain (125 g/ml, Worthington Biomedical, Lakewood, NJ), 100 mM
phosphate buffer, 10mM cystein, 10 mM EDTA, pH 6.3] and digested at 60°C for 16
hours. DNA content was determined by fluorophotometry with Hoechst 33258 dye
(Aldrich). Three samples were harvested for each assay.
