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Fig. 6. Proliferation and differentiation at the midline interfrontal suture in Fgfr2+/S252W mice at E18.5. (A,B) HE staining shows a gap in mutants. (C,D) Ki67 staining shows abnormal distribution of positive cells in the mid-sutural mesenchyme in mutants. (E-H) Expression of osteogenic markers shows displaced, decreased or delayed differentiation in mutants. (E,F) Osteonectin in situ hybridization; (G,H) osteopontin in situ hybridization. (A,C,E,G) Control littermates; (B,D,F,H) corresponding regions in mutant mice. Arrows, osteogenic fronts; s, skin; f, frontal bone; b, brain. Scale bars: A-H, 50 µm.





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