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Fig. 3. Gdf7 fate mapping to the diencephalic and myelencephalic CPe. Gdf7 in situ hybridization (A-B,G-H) and X-gal staining (C-F,I-L) on wild-type or Gdf7Cre;R26R coronal cryosections. Sections in B,D,F are 40-60 µm posterior to those in A,C,E, respectively. (A-F) Diencephalon (3rd ventricle). Gdf7 transcripts mark the E12.5 diencephalic midline and adjacent neuroepithelium (A,B). The E12.5 Gdf7 fate map highlights the same regions as Gdf7 in situ hybridization (C,D), as well as the dCPe at E14.5 (E,F). (G-L) Hindbrain (4th ventricle). Gdf7 transcripts are detected in the mCPe (G,H), and most mCPe cells belong to the Gdf7 fate map (I-L). Prominent labeling is also seen at the junctions between mCPe and adjacent neuroepithelium (cerebellum rostrally, myelencephalon caudally), but little labeling is seen elsewhere at these stages. Scale bars: 0.4 mm in G,I,K; 0.2 mm in A-F,H,J,L.





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