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Fig. 4. Gdf7 cell lineages in the anterior, but not posterior, telencephalic CPe. X-gal stains (A-F,J-L) and Gdf7 in situ hybridization (G-I) on E11.5-14.5 Gdf7Cre;R26R or wild-type coronal cryosections. Semi-serial sections starting at the choroid plaque level are 120 µm (A-F,J-L) or 40-60 µm apart (G-I). Gdf7 cell lineages localize to the choroid plaque (arrowheads), tCPe (arrows) and cortical hem (brackets) in the anterior DMT (A,D,J). No tCPe or hem labeling is detected in the much-larger posterior DMT (B,C,E,F,K-L). In contrast to nearby diencephalic neuroepithelium (asterisks in H,I), Gdf7 mRNA is not detected in the tCPe or hem (arrows in G-I), which suggests true lineage relationships between the Gdf7-expressing DM cells at neural tube stages and the labeled anterior DMT cells at later stages. Scale bar: 0.2 mm.

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