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Fig. 9. Loss of all CPe, including posterior tCPe, after early Gdf7-mediated
ablation. Ttr in situ hybridization and Hematoxylin and Eosin stains on E12.5
and E13.5 coronal cryosections (A-D,G,H) or paraffin sections (E,F), and Ttr
qRT-PCR analysis (I) on E12.5 ACTBCre;Gdf7DTA embryos and controls. (A,B)
Hindbrain. The mutant hindbrain is open and lacks Ttr expression (arrows in
A). (C-I) Forebrain. In the closed mutant forebrain, no Ttr expression (C) or
tissue resembling CP (F,H) is detectable at E12.5 (E) or E13.5 (G). (I) Ttr
qRT-PCR. Ttr transcript levels in the dorsal forebrain are two to three orders
of magnitude lower in the three E12.5 mutants relative to three littermate
controls after normalizing to 18S rRNA (0.004±0.003) or Cyclophilin A
(0.003±0.001). Arrows designate the expected sites of CPe; d, dCPe; m,
mCPe; t, tCPe. Scale bars: 0.2 mm. See Materials and methods for additional
qRT-PCR details.
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