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Fig. 1. tbx2b MO blocks translation of tbx2b and impairs normal
development. (A) RT-PCR using primers specific to the 5'-UTR of
tbx2b shows that this transcript was present as early as 50% epiboly.
(B) Western blot with anti-Myc antibody shows that translation of recombinant
tbx2b-myc was blocked in the presence of tbx2b MO in a
dose-dependent manner. (C) Dorsal view of tbx2b morphant shows the
almost complete loss of shh in the trunk region of the embryo. The
boxed section is enlarged in D, showing the shh-positive cells as
remnants of notochord (arrow). (E) Real-time PCR shows that tbx2b
transcripts are present at a higher level in the ventral gastrula (5.5 hpf)
when compared with EF1
. (F,G) Phenotype of tbx2b
morphant (2 pmol) (G) when compared with control (F) (see text for
description). (H,I) tbx2b is required for proper development of the
eyes and forebrain. (H) Plastic cross-section through the forebrain of a 24
hpf control embryo at the level of the lens. (I) Cross-section of the
tbx2b morphant shows severe disorganization of the forebrain and
eyes.
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