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Fig. 3. Analysis of the late-flowering fy and fca mutants. (A)
Schematic diagram of FY gene and protein and position of fy
mutations. The amino acid changes in fy-3 and fy-4 and
nucleotide change in fy-1 are indicated in parentheses. Exons are
represented as boxes and introns as lines. FY protein is represented as two
protein domains, the WD repeats (7xWD) and C-terminal (CT) domain. (B)
Phenotypes of late-flowering fy and fca mutations with
(+VRN) and without (–VRN) 6-week vernalization treatments at 4°C.
(B) The late-flowering mutants are grown alongside their wild-type, parental
accession, either Ler (fy-1, fca-1) or Col (fy-2, fy-3,
fca-9). (C) Northern blotting and hybridization analysis of FY,
FLC and ß-TUB mRNA expression in wild-type, fy and
fca mutant backgrounds. (D) Western blot analysis of FY accumulation
in wild-type and fy mutant backgrounds. For northern and western blot
analysis RNA and protein were extracted from non-vernalized, 10-day-old
Arabidopsis seedlings.
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