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Fig. 7. Expression pattern of FY. (A-C) FY::GUS expression during seed development. Seeds were histochemically stained for GUS activity which appears as a blue precipitate. Seed were stained at globular (A), heart (B) and mature (C) stages of embryogenesis. Scale bars: 50 µm. (D-F) FY::GUS expression in 12-day-old seedlings. Whole seedlings show GUS staining around the shoot meristem and in young leaves and in the vascular tissue of the cotyledons (D). The roots also show GUS staining in the meristematic regions. In roots, both the root apical meristem (E) and emerging lateral root meristems (F) show GUS expression. (G-J) Expression of the P_FCA–FCA_{to exon5}:GUS reporter transgene in Ler (G), fy-1 (J), fy-2 (I) and fca-1 (H) backgrounds. 12-day-old seedlings were grown on plates and histochemically stained for GUS expression. (K-R) FY expression was confirmed using in situ hybridization. Hybridization with an antisense FY probe to sectioned seed (K-M) or mature seedlings (O-Q) showed expression as blue staining. Scale bars: 50 µm. Hybridization with a sense probe to seeds or mature tissue shows no staining (N and R).

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