The cell adhesion molecule NrCAM is crucial for growth cone behaviour and pathfinding of retinal ganglion cell axons
Development Zelina et al.
132: 3609
DEV01934 Supplementary Material
Files in this Data Supplement:
Movie 1
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Movie 1. DiI-labelled
distal part and growth cone of a RGC axon in a retina flat-mount, cultured in
the presence of non-specific F(ab) fragments. The confocal z-stack (TCS SP2,
Leica) was rendered in 3D and rotated 360° around the x-axis
using Volocity software (Improvision).
Movie 2
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Movie 2. DiI-labelled
distal part of a single RGC axon grown in a retina flat-mount culture in
presence of NrCAM F(ab) fragments. The confocal z-stack (TCS SP2, Leica) was
rendered in 3D and rotated 360° around the x-axis using Volocity
software (Improvision).
Movie 3
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Movie 3.
Time-lapse imaging of DiO-labelled RGC axons in a retina flat-mount. Under
control conditions, axons growing towards the optic fissure (upper right)
mostly have an elongated form and only become more complex during short pauses.
1200-fold accelerated.
Movie 4
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Movie 4. Time-lapse
imaging of DiO-labelled RGC axons in a retina flat-mount. Under NrCAM
inhibition, two axons detach from axon fascicles and turn. Later, one of them
turns back towards the correct direction. 1200-fold accelerated.
