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Fig. 3. Compact elements drive neural Esr gene expression.(A) Promoters of
Esr1, Esr7, Esr10 and Hairy2
(Davis et al., 2001) show high
and moderate homology of S1 and S2, respectively, in the SPS (green). All
exhibit a conserved CCAAT motif (blue). GFP expressed by deletion
mutants of Esr1 (B,C-E) and Esr10 (B,F-H) in transgenic
frogs, followed by whole-mount in situ hybridization, indicates that short
elements drive Esr gene expression in the neural tube (D,G,
arrowheads). Esr10/Dra also drives somitomeric (G, arrow) and tailbud
(G, asterisk) GFP expression. Deletion to a Hin3 (E) site attenuates
Esr1 GFP, although expression remains restricted to neural tissue (E,
arrowhead). Deletion to a Pst site (H) abrogates Esr10 neural
expression, although diffuse somitomeric expression (H, arrow) remains.
Activities using the neural tube (NT) as a reference are summarized in (B,
right; see Table 1 for
details). Sections through the neural tube of stage 20 Esr1/RV
transgenic embryos (J) show GFP-positive cells in the ventricular
zone in a pattern similar to the endogenous gene (I). Also summarized (B,
right) are data reported in Figs
4,
6 and
8 and
Table 2 that are relevant to
responses to ectopic Xngnr1 (NA; not assayed).
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