|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. An intact SPS is required for Esr1 and Esr10 expression.
S1 (I, left) is highly conserved in Esr1, Esr10 and homologous genes,
and matches the optimal RTGRGAR consensus determined by Tun et al.
(Tun et al., 1994). S2 of
Esr1, Esr10 and several E(spl) homologs is less conserved
(mismatches in red). S2 is reported as the bottom strand. Su(H) sites within
the SPS of Esr1 (B,C) and Esr10 (F,G) were mutated
individually (mS1 or mS2) by changing G5 to a C, and GFP expression
in transgenics was monitored by in situ hybridization and compared with
wild-type controls (A,E). Neural and somitomeric Esr10 expression
required two intact Su(H) sites (F,G), while neural Esr1 expression
required only S1 (B,C). Injection of Xngnr1 (ngn; injected side down)
mRNA could not rescue GFP expression in embryos carrying S1 mutations
of Esr1 (Esr1/RvmS1) (D) or Esr10
(Esr10/DramS1) (H). (J) Luciferase activity of HeLa cells transfected
with Esr1/RV SPS mutants showed that whereas mS1 abrogated
transcription, mS2 had no effect.
|
