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Fig. 4. Cytoskeletal remodeling of dendrites during early metamorphosis. (A)
Projected confocal z-stack of ddaC in the abdomen of a wild-type larva
(ppk1.9GAL4> CD8::GFP). The box denotes the region from which
images B-C' were collected. Scale bar: 30 µm. (B,B') The
proximal branch of ddaC at wandering third instar larva stage labeled with
anti-CD8 (B) and anti-Futsch (B'). Arrowhead indicates branches from
another da neuron. (C,C') The proximal branch of ddaC at 3 h APF labeled
with anti-CD8 (C), anti-Futsch (C'); arrows denote beads on branches.
Filopodia (f) are found on branches that have undergone thinning; arrowhead
indicates another da neuron. Scale bar: 10 µm. (D) Dynamics of the
microtubule cytoskeleton of ddaC during the first six hours of metamorphosis.
Projections of z-stacks from a movie showing tub::GFP distribution. 0 h APF
reveals the uniform distribution of tub::GFP. By 2 h APF tubulin is
redistributed resulting in a decrease in some places and an increase (arrow)
in others. By 4 h APF some regions lose significant quantities of tub::GFP
while others accumulate it into beads (arrow). By 6 h APF very large beads
have formed that are separated by lengths of arbor that have little to no GFP
signal. Scale bar: 30 µm.
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