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Fig. 5. Blocking ecdysone signaling in da neurons with EcRDN. (A)
Projected z-stack of wild-type neurons
(C161-GAL4>CD8::GFP) in the dorsal abdomen at 16 h APF.
(B) Projected z-stack of neurons expressing EcRDN
(C161-GAL4>EcRDN; CD8::GFP) in the dorsal
abdomen at 16 h APF. No severed branches are present and only a few blebs of
GFP are evident. Arrows indicate distal dendrites of ddaD and ddaE, arrowheads
the distal dendrites of ddaF. Scale bar: 20 µm. (C) The proximal branches
of dorsal da neurons in a wild-type animal at 16 h APF are thinned. Scale bar:
7 µm. (D) Equivalent proximal branches of dorsal da neurons expressing
EcRDN at 16 h APF show no thinning. Asterisk indicates cell bodies
of ddaF and ddaB still present. (E) Distal region of branch in a wild-type
animal at 16 h APF. Arrows indicate fragments. (F) Distal region of ddaD
expressing EcRDN at 16 h APF. Arrow indicates retraction
bulb. (G) Dynamics of the pruning of dorsal da neurons expressing
EcRDN during early metamorphosis. Arrows indicate branch
retraction. At 24 h APF the dendrite branches are shorter and the retraction
bulbs have increased in size. Branches continue to retract (arrows). Arrowhead
in 25 h 30 min and 26 h 40 min identifies a distal severing event. Scale bar:
20 µm (16 h APF); 25 µm (other times).
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