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Fig. 6. Remodeling of the abdominal epidermis. (A) The position of ddaD and ddaE (yellow arrow/arrowhead) in relation to the larval epidermal cells (pink arrows) and smaller diploid cells of the histoblast nests (pink outline) at 5 h APF in a H2Av::GFP, C161-GAL4>CD8::GFP animal. Scale bar: 45 µm. (B) Removal of larval epidermal cells by phagocytes. Projections of z-stacks from a movie with H2Av::GFP labeling the nuclei of all cells. The boxed region shows the position of the other enlarged images relative to the imaginal histoblast nest (IHN). At 15 h 00 min the larval epidermal cell nucleus is intact (pink arrow), and the small diploid nuclei of phagocytic blood cells (white arrows) are found close by. Between 16 h 40 min and 17 h 20 min the nucleus moves and changes shape, identifying that it has been consumed by a phagocyte. For the remainder of the movie the remnants of the nucleus can be seen inside the phagocyte. Scale bar: 35 µm. (C) Fate of 106 larval epidermal cells during early metamorphosis (14 and 25 h APF). During this time period, 17 larval epidermal cells died (orange dots). Scale bar: 50 µm.

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