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Fig. 8. Phagocyte behavior changes when pruning is blocked. False color indicates the different frames of the respective time-lapse movies; magenta, the first frame and green the final frame. The path of a phagocyte through all of the frames is recorded as a white line, and the final destination denoted with an arrow. (A) In a movie of a wild-type animal (C161-GAL4>CD8::GFP) starting at 0 h (~14 h APF) the phagocyte tracks around the ventral branch of ddaD until it is severed and then moves to the right attacking the retracting branch of ddaE. (B) In a movie of EcRDN expressing neurons (C161-GAL4>EcRDN;CD8::GFP) starting at 0 h (14 h 20 min APF) two phagocytes have been tracked. The phagocyte to the left follows the distal tip of the branch, removing pieces as it retracts. The other phagocyte is found near the proximal arbor and moves slowly across the field and does not interact with the `non-thinned' branches. Scale bar: 30 µm.





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