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Fig. 4. The post-optic glial bridge is disrupted in yot (gli2DR) mutants. Axons (anti-AT; green) and Gfap+ cells (red) at 20 hpf (A,D), 24-25 hpf (B,E), and 36 hpf (C,F) in wild type (WT; A-C) and yot (gli2DR) mutants (D-F). (D-F) POC and RGC axons do not cross the midline in yot (gli2DR) mutants (E,F, arrowheads) (Karlstrom et al., 1999). Gfap+ cells are reduced at the midline in the region where the POC would normally form (right brackets), and spread into the pre-optic area (left brackets). Frontal views, anterior up, dotted line marks the optic recess.





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