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Fig. 4. Defective jba-1D inflorescence stem vasculature and lateral organ
polarity. (A) Cross section through a wild-type stem. (B) Higher magnification
of the boxed region in A. The vascular bundle consists of xylem (xy) located
on the inner side of the bundle and phloem (ph) located on the outer side of
the bundle. (C) Cross section through a jba-1D stem showing
additional vascular bundles at the periphery and ectopic vascular bundles in
the center of the stem. (D,F) Higher magnification of the boxed region in C
and the boxed region in D, respectively. Xylem elements can be detected
ectopically on the outer side of the bundle. (E) Higher magnification of the
vascular bundles located at the center of the jba-1D stem (smaller
box in C). The vascular bundles are radialized, with xylem cells (arrowheads)
surrounding phloem cells. (G,H) Cross section through a radialized
jba-1D leaf. Higher magnification the vascular bundles (H) shows a
polarity in the vascular bundle, with phloem cells at one side of the bundle
and xylem cells protruding to the other side. Scale bars: 500 µm (A,C); 50
µm (B,D,E,H,F); 100 µm (G).
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