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Fig. 5. Elevated expression of miR166 in jba-1D plants. (A)
Schematic of the insertion site of jba-1D relative to annotated
genes. Green triangles indicate the 35S enhancers on the T-DNA vector. The
orange line indicates the region of genomic DNA used to recapitulate the
jba-1D phenotype. (B) Alignment of miR166 with its putative
Arabidopsis class III HD-ZIP gene target sequences. The blue
box indicates the miR166 sequence complementarity to the
HD-ZIP genes sequences. (C) miR166 expression. Blot of low
molecular mass RNA extracted from seedlings and inflorescences of wild-type
and jba plants. The relative amount of miR166 accumulation
in the various tissues is indicated below the autoradiograph. The ethidium
bromide-stained gel is shown as loading control (bottom).
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