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Fig. 1. Embryos from remnants mutant females arrest in early embryogenesis. (A-C) Embryos labeled for DNA (green), microtubules (red) and Cnn to label centrosomes (blue). (A) Wild-type embryo in metaphase of syncytial cycle. (B,C) Embryos from rem^HG24/rem^HG24 arrested with one metaphase spindle (B) and with multiple metaphase spindles (C). Nuclei fail to migrate and accumulate at the anterior of the embryo. Spindles often lack centrosomes and/or are unipolar (insets B,C). (D) Unfertilized egg from rem^RA74/Df(2L)30AC possessing polar bodies but no mitotic spindles. (E,F) Microtubules (white) and FISH against X chromosome (green), and Y chromosome (red). (E) Wild-type embryo in which all mitotic spindles label for both X and Y chromosomes. (F) rem^HG24/rem^HG24 embryo in which mitotic spindles contain both X and Y chromosomes. Scale bars: in A, 20 µm for A,B,C; in D, 50 µm; in E, 10 µm for E,F.

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