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Fig. 6. Drosophila Cks30A interacts with Cdks. Western blots from FLAG-Cks immunoprecipitations probed with antibodies to Cdk1, Cdk2, Cyclin A, Cyclin B and Cyclin B3. The lower band in the Cyclin B lane is antibody heavy chain (HC). FLAG-Cks85A strongly co-immunoprecipitates Cdk1, Cdk2 and the mitotic cyclins, while FLAG-Cks30A co-immunoprecipitates Cdk1 and very weakly Cdk2. Cyclin B and Cyclin B3 are detected only in longer exposures. Cyclin B3 is overexposed here. FLAG-Cks30A^P90A fails to co-immunoprecipitate Cdk1 or mitotic cyclins. Sup (last lane) is IP supernatant.

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