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Fig. 2. Defects in anterior CNS regeneration after Smed-netR RNAi. (A-F)
Eleven-day regenerated cephalic ganglia in control (A-C) and dsRNA-injected
(D-F) animals. In A and D, arrowheads point to the brain's anterior commissure
and the red lines mark the positions of the VNCs. Note the lateral expansion
of the cephalic ganglia in D. (B,E) Nuclear labeling of the same planes as A
and D, respectively, showing the peripheral localization of the neuronal cell
bodies. (C,F) Higher magnification of merged images of A and B, and D and E,
respectively. Arrowhead in C points to a lateral branch projecting from the
cephalic ganglia; the branches are reduced or absent in Smed-netR
dsRNA-injected animals (F). (G-L) Eighteen-day regenerated VNCs in control
(G-I) and dsRNA-injected (J-L) animals. By contrast to the parallel nerve
cords observed in G and H, the Smed-netR dsRNA-injected animals
regenerate a disorganized neural meshwork (J,K). (I,L) Higher magnification of
merged images of G and H, and J and K, respectively. (A,B) Confocal
projections through 10.4 µm. (D,E) Confocal projections through 12 µm.
(C,F) Merge of two single confocal planes. (G-L) Single confocal planes.
Anterior to the left. Scale bar: in L, 100 µm for A-B, D-E, G-H, J-K; 50
µm for C,F,I,L. cg, cephalic ganglia; g, gut; fc, flame cells.
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