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Fig. 3. Nasonia hunchback gene structure and the headless
(hbhl) deletion. (A) Nasonia hunchback gene
structure [GenBank accession numbers: DQ116756 (cDNA), DQ116757 (cDNA),
DQ116758 (genomic)]. Exons are boxed. Arrows indicate putative transcription
start sites. ATG indicates putative initiating methionines. NF1 Zf, MF 1-4 Zf,
and CF1-2 Zf refer to C2H2 zinc fingers, and are indicated as bars. The NF1 Zf
is interrupted by an intron. The HB-GST region, against which the
anti-Nv-Hunchback antibody was raised, is indicated as a stippled box
in exon 4. TAA indicates the stop codon. The shaded box indicates a 3'
UTR. (B) A deletion in Nasonia hunchback in the DNA from
headless (hbhl) mutant embryos. The open reading
frames from headless and wild-type genomic DNA show that the
breakpoints of the 1.497 kb hbhl deletion generate a
frameshift mutation in Nasonia hunchback (GenBank accession number:
DQ116759). The dotted lines on the wild-type Nv hb indicate
contiguous sequence. (C) The alignment of candidate NREs from the 3' UTR
of Nasonia hunchback (Nvhb.1-4) with NREs of D. melanogaster
(Dm), and with candidate NREs from hunchback genes of other insects:
D. virilis (Dv), Tribolium (Tc), Locusta (Lm) and
Schistocerca (Sa), as well as with Dm.cycB1.1.
