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Fig. 7. Fgf3 is sufficient to induce phox2a-positive epibranchial neurons.
Zebrafish embryos were co-injected with HS-fgf3 and HS-gfp
plasmids alone (top) or cas-MO (bottom) at the one-cell stage, heat
shocked for 1 hour at 37°C beginning at 22 hpf and then assayed for
phox2a expression (blue) and gfp expression (red) at 36 hpf.
The majority of ectopic, phox2a-positive neurons (arrowheads) were
immediately adjacent to the gfp-positive cells. Note significantly
increased facial ganglion in cas morphant (bottom left) when compared
with the contralateral control side. Anterior is at left. Scale bar: 50 µm.
Abbreviations are as in Fig.
1.
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