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Fig. 1. Molecular markers of neurogenesis reveal heterogeneity within the
epibranchial ganglia. Lateral views, anterior to the left, showing the pattern
of dorsolateral and epibranchial ganglia at 30 (A,B), 48 (C,D) and 72 (E,F)
hpf. Insets show vibratome sections of epibranchial ganglia from
neurod/
-Hu (C') and phox2b/-Hu
(D') double-stained 48 hpf embryos. (A,C,C',E) In situ
hybridization to detect neurod mRNA in newly specified neural
progenitors (blue), partially overlaps with -Hu staining in
differentiating neurons (brown). (B,D,D',F) Most neural progenitors
co-express phox2b at 30 hpf (B) but expression becomes restricted to
a more proximal populations by 48 and 72 hpf (D,F). (G) Diagram indicating the
spatial relationships of pharyngeal pouches (1-6, black), and three domains of
gene expression within the ganglia (1-3, red). Hu+ proximal neural progenitors
are shown in orange, phox2b+ cells in green (overlap with Hu is
indicated as diagonal hatching) and more distal neurod+ cells in
blue. gAD, dorsal anterior lateral line; gAV, ventral anterior lateral line;
gP, posterior lateral line; gV, trigeminal; gVII, facial (geniculate), gVIII,
auditory; gIX, glossopharyngeal (petrosal); gX, vagal (nodose); ot, otic
placode; tg, trigeminal. Scale bar: 50 µm.
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