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Fig. 6. Early placodal defects in cas/sox32 and snh/bmp7 mutants.
Lateral views, anterior to the left except for in B, which is a transverse
section, and E,F, which are dorsal views. (A,B) Whole-mount in situ
hybridization for foxi1 in embryos at 20 hpf from a cross between
cas+/ heterozygotes reveals no difference between
wild types and mutants. Arrows in B indicate ectodermal expression. (C,D)
foxi1 expression at 48 hpf in both pouch endoderm and epibranchial
placodes in wild type, and loss of expression in cas mutants. (E,F) Dorsal
views showing foxi1 expression at 20 hpf in wild type and severe
reduction of expression in snh/bmp7 mutants. (G,H) Lateral views
showing neurod expression in epibranchial ganglia and severe
reductions in snh/bmp7 mutants. 1-6, pharyngeal pouches; gVII,
facial; gIX, glossopharyngeal; gX, vagal; nt, neural tube; ot, otic
vesicle.