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Fig. 4. Effects of anti-dynein injections on endosome movement in oocytes during
stages 9-10A. Each panel is a projection of eight images from one focal plane
acquired at 20-second intervals. Moving endosomes appear as elongated streaks
while non-moving endosomes appear as circular spots. (A-D) Wild-type oocytes
are shown before injection (A,C) and after injection with antibodies specific
for (B) dynein intermediate chain (anti-DIC) or (D) dynein heavy chain
(anti-Dhc). (E) A wild-type oocyte after injection with nonspecific IgG. (F) A
Khc-null oocyte after injection with anti-DIC. Better views of the
effects of antibody injections are provided in Movies 7 and 8 in the
supplementary material. Scale bar: 25 µm.
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