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Fig. 6. Oocyte microtubule behavior during slow and fast streaming stages. Spinning disk confocal images of (A) a fixed stage 9 oocyte (slow streaming stage) with fluorescence from ${alpha}$ -tubulin antibody staining and of (B) a live stage 9 oocyte with fluorescence from transgenic GFP:: ${alpha}$ -tubulin. Time-lapse movies of GFP fluorescence show that randomly oriented microtubules underwent dynamic churning motions (which can only be seen in Movie 12 in the supplementary material). (C) A fixed stage 10B oocyte (fast streaming stage) with fluorescence from ${alpha}$ -tubulin antibody staining shows large microtubule bundles. (D) A live stage 10B fast streaming oocyte with fluorescence from GFP:: ${alpha}$ -tubulin. Time-lapse movies of the GFP show that microtubules joined in loose, parallel arrays rather than tight bundles. The arrays were dynamic transient structures that aligned and bent with streaming currents (which can only be seen in Movie 13 in the supplementary material). (E) A fixed stage 10B Khc¹⁷ oocyte stained with ${alpha}$ -tubulin antibody that shows some aligned fine filamentous structures, but no large microtubule bundles. Scale bar: 25 µm.

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