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Fig. 1. Whole-mount in situ hybridization to imaginal discs. Wing and leg discs
were hybridized with probes derived from genes predicted to have elevated
expression in wing discs (A-J) or leg discs (A'-J'). (A)
Ect3 probe stained distinct cell clusters in the presumptive dorsal
hinge region of the wing disc; (B) pdm2 probe labeled cells
surrounding the pouch with preferential expression in anterior cells; (C)
Phk-3 probe labeled several domains in the hinge and notum region; (D) CG4914
expression is enriched throughout the wing disc with highest levels in the
dorsal-most region. (E) regucalcin probe produced signal in a
population of hemocytes that are associated with the wing imaginal disc, but
not the leg imaginal discs (E'). Except for Phk-3, which is expressed in
a distinct cell cluster in a ventral position of leg discs, these
`wing-specific' genes did not hybridize at detectable levels to leg discs
(A',B',D',E'). rho, fd96CB, unc-4 and CG7447
generated more intense staining in leg discs
(F',G',H',J'). rho and unc-4 are
also detected in wing discs (F,H), whereas no signal above background could be
detected for fd96Cb and CG7447 in wing discs (G,J). Dorsal is upwards
for all discs; anterior is leftwards.
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