DEV01925 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 - Fig.S1. Acinar-specific recombination of the R26R reporter allele in adult Villin-Cre and Ela-CreERT2 pancreas. (A,B) Pancreas from a 4-month-old bigenic Villin-Cre;R26R mouse was cryopreserved, sectioned, stained for b-gal activity (blue color) and counterstained with Eosin (pink). b-Gal activity is present in acinar cells but not in pancreatic ducts (arrows). Scale bars: 50 mm in A; 10 mm in B. (C-E) Pancreas from 3-month-old bigenic Ela-CreERT2; R26R mouse stained for b-gal activity (blue color) and counterstained with Cy2-conjugated DBA (green fluorescence) to display ductal and endothelial elements. (C,D) Unmerged bright-field and dark-field images. (E,F) Merged images in which a red pseudocolor replaces green fluorescence for clarity. b-Gal activity is found only in DBA-negative acinar cells. Box in E represents area expanded at higher magnification in F.

• Supplemental Figure 2 - Fig.S2. Co-expression of nestin and ductal cytokeratins in explant tissue cultured for 5 days in the presence of TGFa. Separate images are shown for nestin and cytokeratin immunofluorescence. By 5 days in culture, the level of nestin is decreasing but is still present in low amounts (A). Ductal cytokeratins are expressed at high levels at this time point (B).

• Movie 1 - Movie.1. Time lapse video of TGFa-induced acinar-to-ductal metaplasia. High-resolution phase-contrast images of explanted adult pancreatic tissue undergoing acinar-to-ductal metaplasia in response to TGFa are available for viewing as a time-lapse video.