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Fig. 1. Acinar-to-ductal metaplasia in primary explant cultures of mouse pancreas.
(A,B) Phase contrast images of freshly harvested (day 0) non-transgenic
pancreatic explant. (C) Hematoxylin and Eosin-stained section of
non-transgenic day 0 explant. (D) Untreated non-transgenic day 5 explant. (E)
Untreated MT-TGF
day 5 explant cultured in the absence of exogenous
growth factors. (F) Hematoxylin and Eosin-stained section of expanded
duct-like epithelium arising from day 5 MT-TGF pancreas. (G)
Non-transgenic day 5 explant cultured with 50 ng/ml rhTGF. (H)
Non-transgenic day 5 explant cultured with 50 ng/ml rhHGF. (I) Non-transgenic
day 14 explant cultured with 50 ng/ml rhTGF. (J) Quantification of
duct-like structures formed following culture for 5 days with variable
concentrations of either rhHGF (dark bars) or rhTGF (light bars). (K)
Effect of EGF receptor-specific tyrosine kinase inhibitor EKI-785 on expansion
of duct-like epithelium by either 50 ng/ml rhHGF (dark bars) or 50 ng/ml
rhTGF (light bars). Values indicate mean±s.e.m. for three
separate experiments; – – indicates no ductal structures
observed.
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