|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Villin-Cre-based lineage tracing reveals that acinar cells
transdifferentiate into ductal cells. Pancreatic epithelium from
Villin-Cre;R26R mice was isolated and fixed immediately after plating (A,C,E)
or after culture in the presence of TGF
for 5 days (B,D,F). On day 0,
most cells (A) contained ß-gal activity (blue) and were amylase positive
(C) and cytokeratin negative (E), confirming that ß-gal expression was
confined to acinar cells. A small percentage of cells did not display
ß-gal activity, and most of these cells were positive for the ductal
cytokeratins (arrows in C and E). Following 5 days of TGF treatment,
most cells were still ß-gal positive (B). No intact amylase-positive
cells were observed (D) and ß-gal activity was present in cells
expressing ductal cytokeratins (F), indicating that the ß-gal-expressing
acinar cells had transdifferentiated into ductal cells. Scale bars: 100 µm
in A,B; 10 µm in C-F.
|
