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Fig. 6. TGF
-induced acinar-to-ductal metaplasia proceeds through
nestin-positive intermediates. Images from day 0 (A,D,G,J), day 2 (B,E,H,K)
and day 5 (C,F,I,L) TGF-treated non-transgenic pancreatic explants,
double immunolabeled for: (A-C) nestin (green) and amylase (red); (D-F) nestin
(green) and ductal cytokeratins (red). Owing to high cytokeratin and low
nestin expression, composite color is orange rather than yellow (arrowheads);
unmerged images are provided in Fig. S2 in the supplementary material. (G-I)
Ductal cytokeratins (green) and amylase (red); (J-L) carbonic anhydrase II
(green) and amylase (red). Nuclei are labeled in blue. On day 0, there were no
nestin-positive cells and no cells co-expressing acinar and ductal markers.
Following only 2 days of culture, cells had reduced levels of amylase and were
beginning to acquire ductal cytokeratins. Following 5 days of culture, amylase
protein was no longer detected, and cells had acquired expression of both
ductal cytokeratins and carbonic anhydrase II. All images are presented at
identical magnifications.
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