|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Anatomy and histology of strongly affected E18.5 Ndst1 mutant
embryos. (A,C) Wild-type embryo, (B,D) Ndst1 mutant. Gross
examination revealed severe frontonasal dysmorphism, including the lack of
eyes but normal outer ear. (D) Hypoplastic prosencephalon (p). The midbrain
(m), pons (po), medulla (md), the cerebellar primordium (cp), choroid plexus
(chp) and spinal cord (sc) appear normal (sagittal sections). (E-H) Bone (red)
and cartilage (blue) stain of E18.5 embryos. (E,F) Severely affected
Ndst1 mutant embryos show lack of all neural crest cell-derived bones
of the viscerocranium and neurocranium. Some lack or delay of ossification
occurs, especially in the vertebrae and (shortened) limb digits (compare with
wild type, arrow in G), which may be fused (E). The ear capsule (ec) is intact
in these embryos (E,F), as are the occipital (o) and exoccipital (eo) bones,
which are derived from paraxial mesoderm. (G,H) Wild-type littermate controls.
Scale bars: 1 mm.
|
