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Fig. 2. C. elegans lin-4 and let-7 miRNA family members and
3'UTR interactions. (A) Examples of heteroduplexes between
lin-4 and two out of seven binding sites in the lin-14
3'UTR and the single binding site in the lin-28 3'UTR
(Moss et al., 1997;
Wightman et al., 1993). The
duplexes are imperfect and overall duplex structure varies because of binding
site sequence variations; however, the 5' seed region is usually paired.
(B) Representative heteroduplexes demonstrating conservation of
let-7-binding sites in the 3'UTRs of lin-41 homologs
in worm (Ce), flies (Dm) and zebrafish (Zf)
(Pasquinelli et al., 2000;
Slack et al., 2000). (C,D)
Alignments of let-7 and lin-4 family members. Residues
identical to C. elegans let-7 or lin-4 are shown in red. The
5' seed region, which is important for binding-site selection, is
underlined. (C) Alignment of the four worm (Ce) let-7 miRNA family
members with let-7 genes from Drosophila (Dm) and human
(Hs). Only a subset of known human let-7 miRNAs is shown
(Lim et al., 2003). (D)
Alignment of lin-4 miRNA family members
(Ambros et al., 2003;
Lim et al., 2003).
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