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Fig. 6. The Alcian Blue-stained material in the embryonic salivary glands requires papss but not the expression of GAG-related genes. FLP-FRT-mediated recombination and the dominant female-sterile technique were used to generate adult females with germline clones homozygous for papss (A-C), sgl (D-F), sfl (G-I) and frc (J-L). For each of the four genes, embryos lacking both maternal and zygotic expression exhibited a typical segment polarity phenotype (C,F,I,L). Staining with an antibody directed against Windbeutel demonstrated that these embryos nevertheless developed salivary gland tissue (arrowheads in B,E,H,K). The salivary glands of embryos lacking both maternal and zygotic expression of papss failed to stain with Alcian Blue (A), but the salivary glands of embryos lacking maternal and zygotic expression of sgl (D), sfl (G) and frc (J) did stain.





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